Journal: Frontiers in Pharmacology
Article Title: Glomerular Damage in Trichloroethylene-Sensitized Mice: Targeting Cathepsin L-Induced Hyperactive mTOR Signaling
doi: 10.3389/fphar.2021.639878
Figure Lengend Snippet: Loss of podocytes occurred in OMDT patients and TCE-sensitized mice. (A) Expression of urine nephrin before and after clinical treatment in OMDT patients ( n = 6). (B–D) Representative results of immunofluorescence test in mice ( n = 5 per group). (B1–B4) Expression of nephrin in blank control group (B1) , vehicle control group (B2) , TCEpos group (B3) , and TCEneg group (B4) . (C1–C4) Expression of integrin β1 in blank control group (C1) , vehicle control group (C2) , TCEpos group (C3) , and TCEneg group (C4) . (D1–D4) Expression of α-actinin-4 in blank control group (D1) , vehicle control group (D2) , TCEpos group (D3) , and TCEneg group (D4) . (E-G) The fluorescence intensity of nephrin, integrin β1, and α-actinin-4 calculated in Image J software. Data are presented as mean ± SD and determined by paired t -test or one-way ANOVA, * p < 0.05, ns P >0.05; a P < 0.05, vs. blank control group; b P <0.05, vs. vehicle control group; c P <0.05, vs. TCEneg group.
Article Snippet: The human nephrin DuoSet ELISA kit was obtained from R&D System (Minneapolis, United States).
Techniques: Expressing, Immunofluorescence, Control, Fluorescence, Software